The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. Ã- 600 mm long) was produced within a 5 Ã- 30 Ã- 30 mm titanium alloy (Ti-6Al-4V) cuboid, in form of a double handed spiral. The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. Gupta, Vipul Talebi, Mohammad Deverell, Jeremy Sandron, Sara Nesterenko, Pavel N Heery, Brendan Thompson, Fletcher Beirne, Stephen Wallace, Gordon G Paull, Brett These compounds were determined by an automated pre- column derivatization with o-phthalaldehyde-2-mercaptoethanol reagent and UV detection at 338 nm.ģD printed titanium micro- bore columns containing polymer monoliths for reversed-phase liquid chromatography. The selectivity, efficiency and lifetime of normal- and narrow-bore columns for high-performance liquid chromatography were investigated for the separation and quantification of amino acids and the amino acid-like antibiotics phosphinothricin and phosphinothricylalanylalanine in biological samples. Separation of amino acids and antibiotics by narrow-bore and normal- bore high-performance liquid chromatography with pre- column derivatization.
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